- Title
- RNA processing body (P-body) dynamics in mesophyll protoplasts re-initiating cell division
- Creator
- Bhullar, Dilbag S.; Sheahan, Michael B.; Rose, Ray J.
- Relation
- Protoplasma Vol. 254, Issue 4, p. 1627-1637
- Publisher Link
- http://dx.doi.org/10.1007/s00709-016-1053-0
- Publisher
- Springer
- Resource Type
- journal article
- Date
- 2017
- Description
- The ability of plants to regenerate lies in the capacity of differentiated cells to reprogram and re-enter the cell cycle. Reprogramming of cells requires changes in chromatin organisation and gene expression. However, there has been less focus on changes at the post transcription level. We have investigated P-bodies, sites of post transcriptional gene regulation, in plant cell reprogramming in cultured mesophyll protoplasts; by using a YFP-VARICOSE (YFP-VCSc) translational fusion. We showed an early increase in P-body number and volume, followed by a decline, then a subsequent continued increase in P-body number and volume as cell division was initiated and cell proliferation continued. We infer that plant P-bodies have a role to play in reprogramming the mature cell and re-initiating the cell division cycle. The timing of the first phase is consistent with the degredation of messages no longer required, as the cell transits to the division state, and may also be linked to the stress response associated with division induction in cultured cells. The subsequent increase in P-body formation, with partitioning to the daughter cells during the division process, suggests a role in the cell cycle and its re-initiation in daughter cells. P-bodies were shown to be mobile in the cytoplasm and show actin-based motility which facilitates their post-transcriptional role and partitioning to daughter cells.
- Subject
- dedifferentiation; P-bodies; protoplast division; RNA decapping; RNA degredation; RNA storage
- Identifier
- http://hdl.handle.net/1959.13/1397030
- Identifier
- uon:34167
- Identifier
- ISSN:0033-183X
- Rights
- © The Author(s) 2016. This article is published with open access at Springerlink.com. This article is distributed under the terms of the Creative Commons At tribution 4.0 International License (http:/ / creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Language
- eng
- Full Text
- Reviewed
- Hits: 2592
- Visitors: 2708
- Downloads: 156
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 4 MB | Adobe Acrobat PDF | View Details Download |